The Tricarboxylic Acid (TCA) Cycle, also known as the Krebs Cycle or Citric Acid Cycle, is a fundamental metabolic pathway that plays a crucial role in cellular respiration. This cycle is integral to the process by which cells extract energy from carbohydrates, fats, and proteins, making it a cornerstone of Read more…
In the rapidly advancing field of molecular biology, techniques for studying protein interactions are constantly evolving. Among these, Surface Plasmon Resonance (SPR) stands out as a powerful analytical tool. By enabling precise, label-free detection of biomolecular interactions in real time, SPR has become a cornerstone in protein analysis, transforming research and development Read more…
Microscale thermophoresis (MST) has emerged as a cutting-edge analytical technique, offering researchers robust and efficient solutions for studying biomolecular interactions. From drug discovery to protein research, the MST assay is revolutionizing how scientists analyze binding affinities, making it a cornerstone of modern biophysical analysis. This article delves into the principles, Read more…
Nicotinamide adenine dinucleotide (NAD) and its reduced form (NADH) are essential coenzymes involved in a wide array of cellular metabolic processes. As critical mediators in oxidation-reduction (redox) reactions, NAD/NADH plays a pivotal role in energy production and cellular metabolism. Understanding and analyzing these biomolecules has become increasingly important in areas Read more…
Glucosinolates are important secondary metabolites found predominantly in cruciferous vegetables such as broccoli, cabbage, Brussels sprouts, and radish. These sulfur-containing compounds play a dual role in plant biology and human health, making them a fascinating subject of study in fields like agriculture, nutrition, and medicine. Their ability to protect plants Read more…
Proteins are the workhorses of biological systems, performing essential functions ranging from structural support to enzymatic catalysis and cellular communication. Accurate characterization of proteins is crucial for understanding their biological roles in health and disease, as well as for advancing drug development, biotechnology, and diagnostics. Among the diverse methods for Read more…
In the fast-evolving landscape of biotechnology and systems biology, understanding the dynamic processes that sustain life at a cellular level is crucial for advancing research and therapeutic applications. One essential approach to studying cellular metabolism is Metabolic Flux Analysis (MFA), a powerful tool that reveals the intracellular activities and metabolic dynamics Read more…
Exosomes, the small extracellular vesicles secreted by nearly all cell types, have emerged as powerful players in intercellular communication and potential biomarkers for disease diagnosis and therapy. These vesicles are not merely cellular waste products; they are carriers of valuable molecular information, including RNA, lipids, and proteins, which reflect their Read more…
Chromatography is a powerful analytical technique widely used for separating and purifying components within a mixture. The core principle of chromatography involves the movement of a mixture through a stationary phase by a mobile phase. As the components of the mixture travel through the stationary phase, they interact differently with Read more…
What are Exosomes?Exosomes are small extracellular vesicles (EVs) ranging from 30 to 140 nm in diameter. They are crucial carriers of various functional substances, including proteins, non-coding RNAs, mRNAs, and lipids. Acting as important messengers between cells, exosomes can significantly alter the biological activity of recipient cells. The term “exosome” Read more…